Structural formulae are not obligatory.

Paper- I :

1. Molecular and functional organization of a cell and its sub-cellular components.

2. Chemistry of enzymes and their clinical applications.

3. Chemistry and metabolism of proteins and related disorders.

4. Chemistry and metabolism of purines and pyrimidines and related disorders.

5. Chemistry and functions of DNA and RNA, Genetic code, Protein biosynthesis & regulation       (Lac-operon).

6. The principles of genetic engineering and their applications in medicine.

7. Chemistry and Metabolism of haemoglobin.

8. Biological oxidation.

9. Molecular concept of body defence and their applications in medicine.

10. Vitamins and Nutrition.           

Paper – II:

1. Chemistry and metabolism of carbohydrates and related disorders.

2. Chemistry and metabolism of lipids and related disorders.

3. Mineral metabolism: Water and electrolyte balance & imbalance.

4. Acid base balance and imbalance.

5. Integration of various aspects of metabolism and their regulatory pathways.

    Starvation metabolism.

6. Mechanism of hormone action.

7. Environmental biochemistry.

8. Liver function tests, Kidney function tests, Thyroid function tests.

9. Detoxification mechanisms.

10. Biochemical basis of cancer and carcinogenesis.

11. Radioisotopes.

12. Investigation techniques: (LCD-Topics) Colorimeter,

Electrophoresis, Chromatography, Flame photometer & pH measurement.

Group A:

1. Blood Sugar

2. Blood Urea

3. Serum Total Protein, Albumin and A/G ratio

4. Alanine transaminase(SGPT)

5. Aspartate transaminase(SGOT)

6. Alkaline phosphatase

7. Serum amylase

8. Serum total bilirubin

9. Serum uric acid

10. Serum calcium

11. CSF sugar.

12, Serum Creatinine

Group B:

1. Urinary Creatinine

2. Serum cholesterol

3. Serum phosphorus

4. CSF protein

5. Tests for monosaccharides

6. Tests for disaccharides

7. Colour reactions of proteins

8. Precipitation reactions of proteins

9. Normal Organic constituentsofurine

10. Abnormal constituents of urine

Group C (Spots):

1. Identification of osazones

2. Use of reagents.

3. Principle of qualitative tests.

4. Significance of tests.

5. Use of Instruments.

5. Identification of Hb – derivative

6. Identification of GTT, Electrophoretogram and Chromatogram.

TEXT BOOKS:                                                                               

1. Biochemistry for Medical students by D.M.Vasudevan & Shree Kumari.(6^thed.)

2. Medical Biochemistry - U.Satyanarayan.(3^rded.)

3. Textbook of Medical Biochemistry by Rafi MD.(2^nded.)

4. Medical Biochemistry by Debajyoti Das.(14^thed.)

5.Textbook of Medical Biochemistry by Dinesh Puri.(3^rded.)

REFERENCE BOOKS:

1. Harper's Biochemistry.(29^th ed.)

2. Medical Biochemistry by N.V.Bhagwan.(4^thed.)

3. Biochemistry by L.Stryer.(5^thed.)

4. Biochemistry by Orten & Neuhans.(10^thed.)

TERMINAL EXAMINATION  (THEORY & PRACTICAL)

THEORY:

It is usually held immediately after winter (Diwali) vacation. 

It consists of only one paper which will include all topics of paper 1 and paper 2.till the date of the examination.This paper contains Section A, B, & C.

TOTAL MARKS    :  60                                           DURATION  :  2 ½   Hours           

Section A-   Multiple Choice Question -12 Marks

24 MCQ’s x ½ mark each - 12 Marks

Section B – SAQ (Short Answer Question)-24 Marks

a)    Answer any three out of four (3x4 marks)  - 12 marks

b)    Answer any three out of four (3x4 marks)  - 12 marks

Section C – LAQ (Long Answer Question)-24 Marks

Answer any three out of four (3x8 marks)  -  24 marks.

MIDTERM EXAMINATION (Only theory, no practical examination)                      

TOTAL MARKS  : 30                                               DURATION  :  2  Hours

Midterm exam marks are not  included for internal assessment.

It is usually held in the month of January. It consists of only one paper which will include all topics of  Paper1 as well as Paper 2 after terminals till the date of the  examination. This paper contains Section A&B only.                                                        

Section  A- SAQ (Short Answer Question)-12 marks.

Answer any three out of four (3x4 marks) –12 marks

Section B-  LAQ (Long Answer Question)-18 marks.

Answer any two out of three (2 x 9 marks) – 18 marks

PRELIMINARY EXAMINATION –(THEORY & PRACTICAL)

It will be exactly as per MUHS examination pattern.

THEORY   :

 The theory exam in Biochemistry will  consist of two papers.

Paper 1 (50 marks)                                        Duration: 2 ½ hours. 

Paper 2 (50 marks)                                        Duration: 2 ½  hours

Both the papers will contain Section A, B and C.

Section A – MCQ (Multiple Choice Question)-10 marks

Based on single best answer (20MCQ’s x ½ mark each)

Section B – SAQ (Short Answer Question)-24 marks

Answer any six out of seven (6x4marks) ,out of which 2 questions are based on applied  aspects. 

Section C – LAQ (Long Answer Question)-16 marks

Answer any two out of three (2x8marks)

PRACTICALS :Terminal& Preliminary examination

The practical exam pattern for terminal and prelim exam will be the same.  Practical examination in biochemistry will be of two hours duration and will be of 40 marks.

Q.1.     One quantitative experiment from Group A- 20 marks.

            (15 marks for experiment and 5 marks for table viva)

Q.2.     One quantitative/quantitative experiment from Group B-15 marks.

            (10 marks for experiment and 5 marks for table viva)

Q.3.     Spot identification from Group C-(5 spots for one mark each) 

The scheme of MUHS final examinations is stated below-

a)    Theory  Paper I – 50 marks

             Paper II – 50 marks

b)    Oral (viva-voce) – 20 marks

c)    Practicals – 40 marks

d)    Internal assessment - Theory – 20 marks

Practicals -20 marks

INTERNAL ASSESSMENT

THEORY  : 20 Marks

Terminal 60 Marks+ Prelims 100 Marks = Total 160 Marks

Formula for calculation = Marks obtained in terrminals + Prelims

                                                                           8

PRACTICALS : 20 Marks

Terminal 40 Marks+ Prelims 40 Marks = Total 80 Marks

Formula for calculation = Marks obtained in terrminals + Prelims

                                                                       4

Fractional marks obtained in internal assessment are converted to the nearest next integer. e.g. 8.01 to 8.49 marks are rounded off to 8.0 and 8.50 to 8.99 are rounded off to 9.0. 

Standard of passing :

The student must secure at least 35% marks of the total marks fixed for internal assessment in a particular subject in order to be eligible to appear for the final university examination of that subject.  If any student has not secured a minimum of 35% marks in the internal assessment exam, he / she will not be eligible to appear for university Theory, Oral and Practical exams of the respective subject.

ATTENDANCE :

Minimum 75% of attendance for  theory  and 80% for practicals is mandatory, so as to appear for university examination of the respective subject.

LIST OF SPOTS

1.         Identify the given Osazones (Glucosazones / Fructosazones,Maltosazones,Lactosazones)

2.         Identify the given bloodpigment (Oxyhemoglobin 1:200,Oxyhemoglobin 1:25, Reduced hemoglobin, Methemoglobin)

3.         Give the Principle of

            a)         Benedict’s test

            b)         Barfoed’s test

            c)         Fehling’s test

            d)         Nylander’s test

            e)         Seliwanoff’s test

            f)          Rapid furfural test

            g)         Molisch test

            h)        Biuret test

            i)          Xanthoproteic test

            j)          Millon’s test

            k)         Cole’s Aldehyde test

            l)          Sakaguchi test

            m)        Lead Acetate test

            n)        Specific Urease test

            o)         Benzidine test

            p)         Fouchet’s test

            q)         Rothera’s test

            r)          Flame Photometer

4.         State the significance of

a)         Barfoed’stest

b)         Benedict’s test

c)         Seliwanoff’s test

d)         Rapid furfural  test

e)         Benzidine test

f)          Fouchet’s test

g)         Rothera’s test

h)        Gerhardt’s test

i)          Sulphosalicylic acid test

5.         Give the use of

            a)         Urease powder

            b)         Sulphosalicylic acid reagent

            c)         Sulphur powder

            d)         Benzidine powder

            e)         Fouchet’s reagent

            f)          Benedict’s reagent

            g)         Barfoed’s reagent

            h)        Seliwanoff’s reagent

6.         Identify the given spot and state its use

            a)         Cuvette

            b)         Urinometer

            c)         Folin-Wu tube

            d)         Combined Electrode

            e)         Chromatography chamber / Tank

            f)          Horizontal electrophoresis tank

7.         Identify the given instrument and state its use

            a)         Colorimeter

            b)         pH  meter

            c)         Flame photometer

8.         Identify the given technique and state its use

            a)         Ascending paper chromatography

            b)         Circular paper chromatography

            c)         Electrophoresis (Electrophoretogram of plasma proteins)

            9.         Identify the given GTT graph and interprete

            a)         Normal

            b)         Diabetic

10.       Identify the labelled band of the electrophoretogram.